Cyanine5-COOH on molecular probes Cy5-carboxylic acid In situ hybridization PCR probes

Cyanine5-COOH (Cy5-COOH) is a fluorescent dye with a carboxyl functional group that is commonly used in molecular probing applications. Conjugation of Cy5-COOH to a molecule can be achieved by reacting with functional groups such as amino or hydroxyl groups in the molecule. The following are the main applications of Cy5-COOH in molecular probes:

1. In situ hybridization probes:

Conjugating Cy5-COOH to a DNA probe or an RNA probe can be used in in situ hybridization experiments. For example, labeling Cy5-COOH on a DNA probe can be used to detect the distribution of a target DNA sequence in a cell or tissue. This application can be used in gene localization, gene expression analysis, cytogenetic studies and other fields.

2. Fluorescent PCR probes:

Conjugating Cy5-COOH to a PCR primer or probe can be used for fluorescence detection of PCR amplification reactions. For example, labeling Cy5-COOH on a PCR probe can be used to detect the conduct of a PCR reaction and the generation of amplification products by observing an increase in fluorescence signal. This application can be used for gene detection, mutation analysis, single nucleotide polymorphism (SNP) analysis, and so on.

3. Protein labeling probes:

Binding Cy5-COOH to proteins can be used for protein labeling and research. For example, binding Cy5-COOH to tagged proteins, such as GST-tagged proteins, can be used for fluorescent labeling and detection of proteins. This application can help researchers understand protein functions, interactions and regulatory mechanisms.

4. Intracellular molecular probes:

Combining Cy5-COOH with cell-permeable molecules, such as small molecule fluorescent probes, can be used for fluorescent labeling and detection of intracellular molecules. For example, labeling Cy5-COOH on a small molecule probe can be used to observe the localization, distribution and metabolism of intracellular molecules. This application can be used to study cell signaling, drug metabolism and changes in the intracellular environment.

In conclusion, Cyanine5-COOH can be conjugated to molecules such as DNA, RNA, and proteins by reacting with functional groups such as amino or hydroxyl groups in the molecule. This conjugation can be used in several molecular probe applications such as in situ hybridization, PCR amplification, protein labeling and intracellular molecular labeling. By conjugating Cy5-COOH to different molecules, localization, tracking and fluorescent labeling of specific molecules can be achieved, providing an important tool for molecular biology and cellular research.

Related Products:

Cyanine dye CY5  CY5

Cyanine CY5-Amino  CY5-NH2

Cyanine dye CY5-Active ester  CY5-NHS

Cyanine dye CY5-Maleimide  CY5-MAL