Cyanine5-carboxylic acid on cell labeling CY5-COOH amino acid Activity evaluation

Cyanine5-COOH (Cy5-COOH) is a fluorescent dye with a carboxyl functional group that is commonly used in cell labeling applications. Fluorescent labeling of cell membranes can be achieved by reacting with the amino structure in the cell membrane. The following are the main applications of Cy5-COOH for cell labeling:

1. Cellular imaging:

Reacting Cy5-COOH with amino structures in the cell membrane can introduce a fluorescent dye into the cell membrane, thereby realizing fluorescent labeling of the cell. For example, reacting Cy5-COOH with amino lipids in the cell membrane enables fluorescent labeling of the cell membrane. This application can be used in cellular imaging studies to observe the morphology, structure and dynamics of cells.

2. Cell tracking:

Tracking of cells can be achieved by reacting Cy5-COOH with amino structures on the cell membrane. For example, by reacting Cy5-COOH with amino acid residues on the surface of the cell membrane, it is possible to label cells and track their movement and distribution in vivo or in vitro. Such applications can be used in studies of cell migration, cell proliferation and cell signaling.

3. Assessment of cellular activity:

Reacting Cy5-COOH with amino structures on cell membranes enables fluorescent labeling of cells and is used for cell activity assessment. For example, by reacting Cy5-COOH with amino acid residues on the cell membrane, cell activity, health status and metabolic activity can be assessed by observing the intensity and changes in the fluorescent signal. Such applications can be used for cytotoxicity assessment, drug screening and cell function studies.

4. Neuronal synaptic labeling:

Fluorescent labeling of neuronal synapses can be achieved by reacting Cy5-COOH with amino structures on the cell membrane. For example, combining Cy5-COOH with acetylcholinesterase can be used to label neuronal synapses and neurotransmission. This application can be used for the study of neuronal synapse morphology and the resolution of neurotransmission mechanisms.

In conclusion, Cyanine5-COOH can achieve fluorescent labeling of cells by reacting with amino structures in the cell membrane. This labeling can be used in a variety of research fields such as cell imaging, cell tracking, cell activity assessment and neuronal synapse labeling. By reacting Cyanine5-COOH with amino structures in cell membranes, localization, tracking and fluorescent labeling of specific cells can be achieved, providing an important tool for cell biology and neuroscience research.

Related Products:

Cyanine CY5  CY5

Cyanine CY5-Amino  CY5-NH2

Cyanine dye CY5-Active ester  CY5-NHS

Cyanine dye CY5-Maleimide  CY5-MAL