Application of CY5-COOH to bioconjugation Cyanine5-carboxylic group

Cyanine5-COOH (Cy5-COOH) is a fluorescent dye with a carboxyl functional group that is commonly used in bioconjugation applications. Conjugation of Cy5-COOH with biomolecules such as antibodies, proteins, nucleic acids, etc. can be achieved by reacting with functional groups such as amino or hydroxyl groups in biomolecules. The following are the main applications of Cy5-COOH in bioconjugation:

1. Antibody labeling:

Conjugating Cy5-COOH to an antibody can be used for immunofluorescence to detect the expression and localization of specific proteins. For example, conjugating Cy5-COOH to an antibody can be used for immunohistochemistry (IHC) or immunofluorescence staining to observe the distribution of a target protein in cells or tissues. Such applications can help researchers understand the localization of proteins, their expression levels, and changes associated with disease.

2. Nucleic acid probes:

Conjugating Cy5-COOH to DNA/RNA molecules can be used for fluorescence detection in fluorescence in situ hybridization, PCR amplification, and so on. For example, labeling Cy5-COOH on a DNA probe can be used in in situ hybridization experiments to observe the distribution of target sequences in cells or tissues. This application can be used in gene detection, gene expression analysis, single-cell analysis and other fields.

3. Protein labeling:

Conjugating Cy5-COOH to proteins can be used for fluorescent labeling and studies of proteins. For example, conjugation of Cy5-COOH to labeled proteins (e.g., GST-tagged proteins) can be used for enzyme activity detection and protein interaction studies. Such applications can help researchers understand protein functions, interactions and regulatory mechanisms.

In conclusion, Cyanine5-COOH can be conjugated to biomolecules such as antibodies, cell membranes, DNA/RNA and other biomolecules by reacting with functional groups such as amino or hydroxyl groups in biomolecules. This conjugation can be used in several biological research fields such as immunofluorescence detection, cell labeling, and protein labeling. By conjugating Cy5-COOH to different biomolecules, localization, tracking and detection of specific molecules can be achieved, providing an important tool for biological research and clinical applications.

Related Products:

Cyanine CY5  CY5

Cyanine CY5-Amino  CY5-NH2

Cyanine dye CY5-Active ester  CY5-NHS

Cyanine dye CY5-Maleimide  CY5-MAL