Structure and properties of CY5-COOH Cyanine5-carboxylic group

Cyanine5-COOH (Cy5-COOH) is a derivative of Cyanine5 that adds a carboxylic acid (COOH) group to the structure of Cyanine5. Below are some descriptions of the structure and properties of Cyanine5-COOH:

1. Structure: The structure of Cyanine5-COOH is essentially the same as that of Cyanine5, and contains an organic dipolar backbone consisting of two linked azacycles connected by a five-carbon chain. The ends of this backbone are usually connected with electron-attracting groups, such as indole rings. In Cy5-COOH, there is an additional carboxylic acid group that allows it to form covalent bonds with biomolecules (e.g., proteins, antibodies, etc.).

2. Nature:

   - Fluorescent Properties: Cy5-COOH has a maximum absorption wavelength of approximately 650 nm and a maximum emission wavelength of approximately 670 nm.This fluorescent property in the near infrared region makes Cy5-COOH particularly useful in bioimaging, where the autofluorescent background of biological tissues is low in the wavelength region and light penetration is good.

   - Chemical reactivity: The carboxylic acid group in Cy5-COOH can form covalent bonds with biomolecules through esterification or amidation reactions. In addition, the carboxylic acid group also makes Cy5-COOH somewhat hydrophilic, improving its solubility in water or aqueous solvents.

   - Stability: Cy5-COOH has good photostability and chemical stability and can maintain its fluorescent properties under prolonged exposure. However, Cy5-COOH is sensitive to oxygen and pH, so it needs to be used and stored in an oxygen-free and neutral pH environment.

   - Solubility: Compared to Cyanine5, Cy5-COOH may be slightly less soluble in organic solvents such as DMSO, but more soluble in water or aqueous solvents.

Related Products:

Cyanine CY5  CY5

Cyanine CY5-Amino  CY5-NH2

Cyanine dye CY5-Active ester  CY5-NHS

Cyanine dye CY5-Maleimide  CY5-MAL