What is the difference between CY3-PEG-NH2, CY5-PEG-NH2 and CY7-PEG-NH2 applications? MW:3400 Fluorescence wavelength Fluorescence intensity

CY3-PEG-NH2, CY5-PEG-NH2 and CY7-PEG-NH2 are three compounds with different fluorescence properties, and the differences between them are mainly in fluorescence wavelength and fluorescence intensity.

First, CY3-PEG-NH2 is a compound containing CY3 fluorescent dye and PEG.CY3 fluorescent dye emits fluorescence in the visible spectral range, with a maximum emission wavelength of about 570 nm.Therefore, CY3-PEG-NH2 is often used for the red channel in microscope imaging and fluorescence detection. Its moderate fluorescence intensity makes it suitable for general fluorescence experiments.

Second, CY5-PEG-NH2 contains CY5 fluorescent dye and PEG.CY5 fluorescent dye has a maximum emission wavelength of about 670 nm, and it emits fluorescence in the infrared region. Therefore, CY5-PEG-NH2 is commonly used for infrared fluorescence imaging and detection. Compared to CY3-PEG-NH2, CY5-PEG-NH2 has a higher fluorescence intensity and is suitable for experiments requiring higher sensitivity.

Finally, CY7-PEG-NH2 contains CY7 fluorescent dye and PEG.CY7 fluorescent dye has a maximum emission wavelength of about 750 nm, which belongs to the near-infrared region.CY7-PEG-NH2 is commonly used for near-infrared fluorescence imaging and detection, and is particularly suitable for deep tissue imaging.The fluorescence intensity of CY7-PEG-NH2 is high, and it has a good signal-to-noise ratio.

In summary, CY3-PEG-NH2, CY5-PEG-NH2 and CY7-PEG-NH2 differ in fluorescence wavelength and fluorescence intensity, which are suitable for different fluorescence experiments and application scenarios. Researchers can choose suitable compounds for fluorescence labeling and fluorescence detection according to their experimental needs.