CY3-Polyethylene Glycol-Carboxylate Application CY3-PEG-COOH MW:5000 Fluorescent Probe Connector Amino Reactivity

CY3-PEG-COOH is a compound containing a CY3 fluorescent dye and a carboxyl group, which has a wide range of applications in biomolecular labeling and bioanalysis.

For biomolecular labeling, CY3-PEG-COOH can covalently bind to biomolecules (e.g., proteins, peptides, antibodies, etc.) that are amino-reactive. By reacting with the amino groups in these biomolecules, CY3-PEG-COOH can be attached to their surfaces to achieve fluorescent labeling. Doing so enables visual tracking, localization and quantitative analysis of biomolecules. For example, binding CY3-PEG-COOH to proteins can be used for fluorescent immunostaining to visualize the distribution of proteins in cells or tissues.

In bioanalysis, CY3-PEG-COOH can be used in fluorescence immunoassay, fluorescence in situ hybridization, fluorescent dye-labeled in situ PCR and other techniques. By combining CY3-PEG-COOH with target molecules (e.g., DNA, RNA, etc.), fluorescence detection and quantitative analysis of them can be realized. For example, labeling CY3-PEG-COOH onto a DNA probe can be used in in situ hybridization techniques to observe the distribution of the target DNA in the cell nucleus by fluorescence microscopy, thereby studying biological processes such as gene expression and mutation.

In addition, since CY3-PEG-COOH contains carboxyl groups, it can also be used as a connector of fluorescent probes to other molecules. For example, combining CY3-PEG-COOH with nanoparticles or fluorescent probes can form nanoprobes with fluorescent signals for bioimaging and biosensor research.

In summary, CY3-PEG-COOH has a wide range of applications in biomolecular labeling and bioanalysis, and can be used in fluorescent labeling, fluorescence immunoassay, fluorescence in situ hybridization and other experiments and techniques.