Application of RB-PEG-SH Rhodamine-polyethylene glycol-mercapto MW:2000 Mercaptan reactivity Disulfide bond Quantitative analysis

Rhodamine-PEG-SH is a compound with fluorescent properties and sulfhydryl functional groups, which has a wide range of applications in biomolecular labeling and biomaterial modification.

For biomolecular labeling, Rhodamine-PEG-SH can react with molecules with sulfhydryl reactivity to form stable disulfide bonds. Through this reaction, Rhodamine-PEG-SH can be attached to proteins, peptides, antibodies, and other biomolecules containing sulfhydryl groups, and fluorescent labeling of these molecules can be realized. For example, by reacting Rhodamine-PEG-SH with antibodies containing sulfhydryl groups, fluorescently labeled antibodies can be prepared for use in fields such as immunofluorescence staining or flow cytometry.

For biomaterial modification, Rhodamine-PEG-SH can be used to modify the surface of biomaterials with sulfhydryl reactivity. By reacting Rhodamine-PEG-SH with sulfhydryl groups on the surface of biomaterials, fluorescent labeling or functionalized modification of biomaterials can be achieved. For example, by modifying Rhodamine-PEG-SH on the surface of gold nanoparticles, nanoprobes with fluorescent signals can be prepared for biosensing, intracellular labeling, and other applications.

In addition, Rhodamine-PEG-SH can be used in areas such as quantitative analysis of biomolecules and fluorescence microscopy imaging. By binding Rhodamine-PEG-SH to the target molecule, quantitative determination of the target molecule can be realized. Meanwhile, due to the fluorescent nature of Rhodamine-PEG-SH, it can be used in fluorescence microscopy to observe the distribution and localization of target molecules in cells or tissues.

In summary, Rhodamine-PEG-SH has a wide range of applications in biomolecular labeling and biomaterial modification, and can be used in the fields of fluorescent labeling, biomaterial modification, quantitative analysis and fluorescence microscopy imaging.