Applications of Cyanine5-NH2 CY5-NH2 Bioimaging Pharmacokinetic studies

As a dye with fluorescent properties, CY5-NH2 is widely used in biological research, biomedical imaging and drug analysis. The following are the main applications of CY5-NH2 :

1. Biomolecular labeling and imaging:

CY5-NH2 can be used to label and visualize a variety of biomolecules such as proteins, antibodies, nucleic acids and cells. For example, labeling CY5-NH2 on an antibody can be used for immunohistochemistry, immunofluorescence staining and flow cytometry. Detection of the localization and expression of a particular biomolecule in a cell or tissue can be achieved by the fluorescent signal of CY5-NH2.

2. Molecular biology and cellular imaging:

Labeling CY5-NH2 on nucleic acid probes or biotags can be used in molecular biology and cellular imaging. For example, labeling CY5-NH2 on DNA or RNA probes can be used for in situ hybridization, FISH (fluorescence in situ hybridization) and real-time PCR. The fluorescent signal of CY5-NH2 enables the study of gene expression, DNA/RNA localization and cellular function.

3. Biomedical imaging:

CY5-NH2 can be used for biomedical imaging, such as tumor imaging, vascular imaging and lymph node imaging. For example, by labeling CY5-NH2 on a tumor-specific antibody or small molecule targeting probe, localization and imaging of tumors can be achieved by injecting the probe and using a near-infrared laser for excitation. Such applications can be used for tumor diagnosis, therapeutic monitoring and drug discovery.

4. Drug analysis and pharmacokinetic studies:

Labeling CY5-NH2 on drug molecules can be used for drug analysis and pharmacokinetic studies. For example, labeling CY5-NH2 on a drug molecule can enable drug content determination, metabolic process studies and drug distribution monitoring in the body by fluorescence detection.

In conclusion, CY5-NH2 is widely used in the fields of biomolecular labeling, imaging, molecular biology, cellular imaging, biomedical imaging, drug analysis and pharmacokinetic studies. By labeling CY5-NH2 on specific biomolecules or drugs, it is possible to study and monitor biological processes, disease development and drug effects. Such applications can help researchers understand the function of biological systems and the mechanism of action of drugs, thus providing powerful tools and methods for life science research and drug development.