CY3-NHS on immunofluorescence techniques Cyanine3-active ester Immunohistochemical staining

Cyanine3-NHS (CY3-NHS) is often used in immunofluorescence techniques to label antibodies for the detection and localization of specific antigens or cell surface molecules. The following are applications of CY3-NHS in immunofluorescence techniques:

1. Immunohistochemical staining:

CY3-NHS can be reacted with an amino group in an antibody for immunohistochemical staining. For example, in immunohistochemical staining, CY3-NHS can be conjugated to a specific primary antibody, and then this labeled primary antibody can be conjugated to the corresponding antigen in the tissue section to be tested. By detecting the fluorescent signal of CY3-NHS, the distribution and localization of the antigen in the tissue section can be visualized and quantified.

2. Immunocyte staining:

CY3-NHS can be conjugated to cell surface antigens or markers for use in immunocyte staining. For example, in immunocyte staining, CY3-NHS can be conjugated to a specific primary antibody, and then that labeled primary antibody can be conjugated to a cell surface antigen or marker to be tested. By detecting the fluorescent signal of CY3-NHS, the presence and localization of the cell surface antigen or marker can be visualized and quantified.

3. Immunofluorescence microscopy imaging:

CY3-NHS-labeled antibodies can bind to antigens in the sample to be tested and be observed and analyzed by immunofluorescence microscopy imaging. For example, in cell culture, the CY3-NHS-labeled antibody can be added to a sample to bind to a target antigen, and then observed and analyzed by immunofluorescence microscopy imaging to observe and analyze the intracellular distribution and localization of the antigen.

In conclusion, CY3-NHS has important applications as a marker in immunofluorescence technology in immunohistochemical staining, immunocytostaining and immunofluorescence microscopy imaging. By reacting with amino groups in antibodies, CY3-NHS achieves fluorescent labeling of specific antigens or cell surface molecules for their localization, visualization and quantitative analysis. These applications provide important tools for biological research, diagnostics and drug development.