Cyanine3-active ester for biomarker applications CY3-NHS

Cyanine3-NHS (CY3-NHS) is an activated fluorescent dye that reacts with amino groups in biomolecules for biomarkers and fluorescence microscopy imaging. The following are applications of CY3-NHS for biomarkers:

1. Protein labeling:

CY3-NHS can react with amino groups in proteins to achieve fluorescent labeling of proteins. For example, in immunofluorescence staining, CY3-NHS can be conjugated to antibodies, and then these labeled antibodies can be conjugated to specific proteins in the sample to be tested. By detecting the fluorescent signal of CY3-NHS, the presence and localization of the target protein can be visualized and quantified.

2. Cell labeling:

CY3-NHS can react with amino groups on the cell surface to achieve fluorescent labeling of cells. For example, in cellular imaging studies, CY3-NHS can be conjugated to a specific protein on the cell surface and used to visualize and track the distribution and localization of that protein within the cell. This allows for the study of cell structure and function, as well as the dynamics of specific proteins in the cell.

3. Organizational markers:

CY3-NHS can react with amino groups in tissues to achieve fluorescent labeling of tissues. For example, in tissue section imaging, CY3-NHS can be conjugated to specific antibodies, and these labeled antibodies can then be conjugated to specific cell types or structures in the tissue section. By detecting the fluorescent signal of CY3-NHS, the presence and localization of the specific cell type or structure in the tissue can be visualized and quantified.

In conclusion, the applications of CY3-NHS as an activated fluorescent dye in biomarkers mainly include protein labeling, cell labeling and tissue labeling. By reacting with amino groups in biomolecules, CY3-NHS enables fluorescent labeling of biomolecules, cells and tissues, as well as fluorescence microscopy imaging. These applications provide important tools for cell biology, molecular biology and histology research.