Cyanine dye CY3-carboxy on immunofluorescence techniques Cyanine 3-COOH fluorescent dye Labeled antibodies

CY3-COOH has a wide range of applications in immunofluorescence technology. Immunofluorescence technology is a commonly used biological assay that allows for the detection and visualization of specific antigens by labeling antibodies with fluorescent dyes. The following are applications of CY3-COOH in immunofluorescence:

1. Immunohistochemistry:

CY3-COOH can be used to label antibodies for immunohistochemical staining. For example, by labeling CY3-COOH on a primary antibody, the signal can be observed by fluorescence microscopy and the localization of the target antigen can be determined by specific binding to the target antigen. This method is commonly used to study the expression and localization of specific proteins in tissues.

2. Immunocyte staining:

CY3-COOH can be used to label antibodies for immune cell staining. For example, in immunofluorescence flow cytometry, a CY3-COOH-labeled antibody is conjugated to a specific antigen on the surface of a cell, and by detecting the fluorescent signal of CY3-COOH, the expression level of the antigen on the surface of the cell can be quantitatively analyzed.

3. Immunohistological analysis:

CY3-COOH can be used to label antibodies for immunohistological analysis. For example, by combining CY3-COOH-labeled antibodies with techniques such as immunoblotting, it is possible to qualitatively and quantitatively analyze the expression levels of specific proteins in different tissues or cells.

4. Immunodiagnosis:

CY3-COOH can be used to label antibodies for immunodiagnostic purposes. For example, labeling CY3-COOH on a primary antibody can detect the presence and concentration of a target antigen in an in vitro diagnostic kit for use in disease diagnosis and monitoring by binding to a specific antigen.

In conclusion, CY3-COOH, as a fluorescent dye, has a wide range of applications in immunofluorescence technology. By labeling CY3-COOH on antibodies, the detection and visualization of specific antigens can be achieved, which provides a powerful tool for the fields of immunohistochemistry, immunocyte staining, immunohistological analysis and immunodiagnosis.