Cyanine 3-COOH in Biomarkers Cyanine dye CY3-carboxylic group Visualization

CY3-COOH, as a fluorescent dye, has a wide range of applications in biomarkers. By covalently linking CY3-COOH to biomolecules (e.g., proteins, antibodies, nucleic acids, etc.), labeling and visualization of these biomolecules can be achieved. The following are the applications of CY3-COOH in biomarkers:

1. Protein labeling:

CY3-COOH can react with amino groups, hydroxyl groups or other functional groups in proteins to achieve labeling of proteins. For example, CY3-COOH can be reacted with an amino group in a protein to form a covalently bound protein-CY3-COOH complex. In this way, the target protein can be localized and visualized by detecting the fluorescent signal of the CY3-COOH.

2. Antibody labeling:

CY3-COOH can be reacted with an amino group, a hydroxyl group, or other functional groups in the antibody to achieve labeling of the antibody. For example, CY3-COOH can be reacted with the amino group in the antibody to form a covalently bound antibody-CY3-COOH complex. In this way, the interaction of the target antibody and antigen can be localized and visualized by detecting the fluorescent signal of CY3-COOH.

3. Nucleic acid labeling:

CY3-COOH can be reacted with amino, hydroxyl or other functional groups in DNA or RNA to achieve labeling of nucleic acids. For example, CY3-COOH can be reacted with an amino group in DNA to form a covalently bound DNA-CY3-COOH complex. In this way, a target DNA or RNA sequence can be localized and visualized by detecting the fluorescent signal of the CY3-COOH.

4. Cell labeling:

CY3-COOH can be reacted with proteins or other functional groups on the cell surface to achieve labeling of the cell. For example, CY3-COOH can be reacted with amino groups on the cell surface to form a covalently bound cell-CY3-COOH complex. In this way, the target cell can be localized and visualized by detecting the fluorescent signal of the CY3-COOH.

In conclusion, CY3-COOH, as a fluorescent dye, has a wide range of applications in biomarkers. By covalently linking with biomolecules, labeling and visualization of proteins, antibodies, nucleic acids and cells can be achieved, providing a powerful tool for biological research and fluorescence imaging.