Application of CY3 to DNA sequencing

The cyanine dye CY3 (Cyanine 3) is also used in DNA sequencing, a technique used to determine DNA sequences by labeling DNA fragments with CY3. The following are applications of Cyanine dye CY3 in DNA sequencing and corresponding examples:

1. Dye-terminator sequencing:

In Dye-terminator sequencing, each base is labeled with a different fluorescent dye, including CY3. By using CY3-containing ddNTP (dideoxynucleotide triphosphate) in the sequencing reaction, CY3 can be labeled at the terminating base position on the DNA fragment. Subsequently, the fluorescently labeled DNA fragments are separated by electrophoresis or other methods that allow the fluorescent signal to be read and the DNA sequence to be determined. For example, labeling CY3 on ddATP can be used to label the A termination site on a DNA fragment in a sequencing reaction.

2. Fluorescence in situ hybridization (FISH):

FISH is a technique that uses fluorescently labeled probes to localize chromosomes or specific DNA sequences. By labeling CY3 on a FISH probe, the CY3-labeled probe can be hybridized to a target DNA sequence, which can then be observed and localized to the location of the target DNA sequence. For example, labeling a CY3 on a chromosome specific probe can be used to localize a specific gene or DNA sequence on a chromosome.

3. Next-Generation Sequencing (NGS):

In NGS sequencing, sequencing of DNA fragments can be achieved by labeling CY3 on an adaptor (adapter) or primer (primer). CY3 labeling on the adapter or primer can be used to detect the ligation or amplification of DNA fragments. For example, in Illumina sequencing, the CY3 marker on the aptamer can be used to detect the efficiency and quality of aptamer ligation.

In conclusion, applications of the cyanine dye CY3 in DNA sequencing include Dye-terminator sequencing, fluorescence in situ hybridization (FISH) and Next-Generation Sequencing (NGS) sequencing. By labeling CY3 on DNA fragments, probes or aptamers, detection, localization and sequencing of DNA sequences can be achieved, providing a powerful tool for DNA research and genomics studies.