CY3 on molecular probes Molecular labeling Visualization

The flower cyanine dye CY3 (Cyanine 3) also has a wide range of applications in molecular probes. Molecular probes are a tool used to detect and study the interactions and dynamics of biomolecules. Labeling and visualization of biomolecules can be achieved by labeling CY3 on nucleic acid probes or protein probes. The following are applications of the flower cyanine dye CY3 in molecular probes and corresponding examples:

1. DNA or RNA probes:

Labeling CY3 on a DNA or RNA probe can be used to detect and localize specific sequences of DNA or RNA. e.g., labeling CY3 on a DNA probe can be used to detect specific sequences of genes and to observe gene expression and localization. Observation of the stained cells or tissues by fluorescence microscopy can provide information on the spatial distribution and amount of the target DNA or RNA.

2. Protein probes:

Labeling CY3 on a protein probe can be used to detect and localize the expression and distribution of specific proteins. For example, labeling and visualization of proteins can be achieved by labeling CY3 on an antibody that binds to a specific protein. Observation of the stained cells or tissues by fluorescence microscopy can provide information on the spatial distribution and localization of the protein in the cells or tissues.

3. Protein-protein interaction probes:

Labeling CY3 on a protein probe is used to study protein-protein interactions. For example, CY3 is labeled on protein A, another protein B is labeled on a fluorescent dye, the two labeled proteins are mixed, and their interactions are observed. Observation of the stained cells or tissues by fluorescence microscopy provides information on the spatial distribution of protein-protein interactions.

In conclusion, the applications of the cyanine dye CY3 in molecular probes include DNA or RNA probes, protein probes and protein-protein interaction probes. By labeling CY3 on probes, labeling and visualization of specific molecules can be achieved, providing a powerful tool for molecular biology research and life science research.