Structure and properties of CY5.5-COOH Cyanine5.5-carboxylate

Cyanine 5.5-COOH is a fluorescent dye commonly used in biomarker and fluorescent staining experiments. It is an azo compound with an azo group and a carboxyl group.

The structure of Cyanine5.5-COOH is shown below:

CH3(CH2)2N(CH3)2 N=N C6H4CH=CHCOOH

Its chemical name is indocarbocyanine-5.5-carboxylic acid.Cyanine5.5-COOH is a near-infrared fluorescent dye with a long wavelength and low background signal.

Key properties of Cyanine5.5-COOH include:

1. Fluorescence properties: Cyanine 5.5-COOH has maximum absorption and emission peaks at light in the wavelength range of 675-694 nm, so it can be used for near-infrared fluorescence staining and imaging.

2. Solubility: Cyanine 5.5-COOH is readily soluble in some organic solvents such as DMSO and methanol.

3. Chemical reactivity: The carboxyl group in Cyanine 5.5-COOH has high reactivity and can condense with substances containing amino or hydroxyl groups to form stable amide or ester bonds.

4. Biocompatibility: Cyanine 5.5-COOH is relatively friendly to living organisms and can be labeled in cells and tissues for fluorescence microscopy observation and analysis.

In conclusion, Cyanine5.5-COOH is a commonly used near-infrared fluorescent dye with good fluorescence properties and chemical reactivity for biomarker and fluorescent staining experiments.