Flower Cyanine Dye CY5.5-Active Ester Applications Cyanine5.5-NHS Bioimaging Fluorescent Labeling

Cyanine5.5-NHS (Cy5.5-NHS) is a fluorescent dye with the property of emitting high intensity fluorescence in the near infrared region. It has a wide range of applications in biomedical research, here are some specific applications :

1. Biomolecule labeling: Cy5.5-NHS can fluorescently label biomolecules by forming covalent bonds with amino groups in biomolecules. For example, researchers can use Cy5.5-NHS to label antibodies, which can then be used to detect and localize antigens in cells or tissues.

2. Immunofluorescence: In immunofluorescence, antibodies labeled with Cy5.5-NHS can be used to identify and localize specific antigens. For example, Cy5.5-NHS can be labeled onto an antibody and this antibody can then be used to detect tumor markers in cells.

3. Biological imaging: Cy5.5-NHS can be used for imaging in living organisms, especially for imaging deep tissues. For example, Cy5.5-NHS can be labeled onto specific probes, and then such probes can be injected into animals, and the distribution and dynamics of the probes in the body can be observed by fluorescence imaging.

4. Fluorescence resonance energy transfer (FRET): Cy5.5-NHS can act as a donor or acceptor for FRET. For example, researchers can label Cy5.5-NHS and another fluorescent dye onto two proteins that interact with each other, and then study the interaction of the two proteins by observing the FRET signal.

5. Flow cytometry: Cy5.5-NHS can be used in flow cytometry to sort and analyze cells. For example, a researcher can label Cy5.5-NHS onto a specific antibody and then use this antibody to label cells and analyze the labeled cells by flow cytometry.

6. DNA/RNA probes: Cy5.5-NHS can be used as a fluorescent marker for DNA or RNA probes to detect and study gene expression and regulation. For example, Cy5.5-NHS can be labeled onto a DNA probe, which can then be used to probe specific gene sequences.

7. Drug screening and drug carriers: Cy5.5-NHS can be used to label drugs to track their distribution and metabolism in the body. For example, Cy5.5-NHS can be labeled onto a drug molecule, and then the distribution and dynamics of the drug in the body can be observed by fluorescence imaging.