Cyanine5-Mal Applications CY5-Maleimide Protein Covalent Labeling Biosensors

Cyanine5-Mal has a wide range of applications in the biological sciences and is commonly used in experiments such as biomarkers, fluorescent staining and fluorescent imaging. Here are some applications of Cyanine5-Mal :

1. Protein covalent labeling: Cyanine5-Mal can covalently bind to proteins containing sulfhydryl groups for protein localization and expression analysis. For example, Cyanine5-Mal can be reacted with antibodies or proteins containing sulfhydryl groups to form stable thioether bonds for fluorescent immunostaining or protein labeling experiments.

2. Cell tracing: Cyanine5-Mal can be used in cell tracking experiments to study processes such as cell migration, proliferation and differentiation. For example, Cyanine5-Mal is reacted with intracellular sulfhydryl groups to form stable thioether bonds, which are labeled on the surface or inside of the cell and used to track the movement of the cell.

3. Drug delivery studies: Cyanine5-Mal can be used to study drug delivery and release processes. For example, Cyanine5-Mal is labeled on nanoparticles or drug delivery systems and is used to track drug distribution and release kinetics in vivo by binding to drugs containing sulfhydryl groups.

4. Protein interaction studies: Cyanine5-Mal can be used to study protein interactions with other molecules. For example, Cyanine5-Mal is labeled on proteins for use in fluorescence resonance energy transfer (FRET) experiments to study interactions between proteins and ligands, enzymes or subunits.

5. Biosensor research: Cyanine5-Mal can be used to construct biosensors for the detection and measurement of target molecules in biological samples. For example, Cyanine5-Mal is labeled on biosensors for the detection of signaling molecules, metabolites, or proteins in cells.

In conclusion, Cyanine5-Mal has a wide range of applications in biosciences and can be used in experiments such as protein covalent labeling, cellular tracing, drug delivery studies, protein interaction studies and biosensor studies.