Structure and properties of CY5-MAL Cyanine5-MAL

Cyanine5-Mal is a fluorescent dye commonly used in biomarker and fluorescent staining experiments. It is an azo compound with an azo group and a maleylidene group.

The structure of Cyanine5-Mal is shown below:

CH3(CH2)2N(CH3)2 N=N C6H4CH=CHCOOH

It is chemically known as N-maleimide of indocarbocyanine.Cyanine5-Mal is a fluorescent dye that absorbs and emits red light with high fluorescence quantum yield and photostability.

Key properties of Cyanine5-Mal include:

1. Fluorescent properties: Cyanine5-Mal has maximum absorption and emission peaks at light in the wavelength range of 620-650 nanometers, so it can be used for red fluorescent staining.

2. Solubility: Cyanine5-Mal is readily soluble in a number of organic solvents such as DMSO and methanol.

3. Chemical reactivity: The maleoyl substituent group in Cyanine5-Mal is highly reactive and can condense with substances containing sulfhydryl groups to form stable thioether bonds.

4. Biocompatibility: Cyanine 5-Mal is relatively biologically friendly and can be labeled in cells and tissues for fluorescence microscopy observation and analysis.

In conclusion, Cyanine5-Mal is a commonly used red fluorescent dye with good fluorescent properties and chemical reactivity for biomarker and fluorescent staining experiments.