Applications of Cyanine5-NHS CY5-active ester Fluorescent staining Cell tracking

Cyanine5-NHS has a wide range of applications in the field of biological sciences and is commonly used in experiments such as biomarkers, fluorescent staining and fluorescent imaging. Below are some application areas of Cyanine5-NHS and corresponding examples:

1. Immunofluorescence staining: Cyanine5-NHS can be conjugated with antibodies for immunocell staining and protein localization experiments. For example, in immunohistochemical analysis, Cyanine5-NHS can be labeled on specific antibodies and used to detect target proteins in cells.

2. Cell tracing: Cyanine5-NHS can be used in cell tracking experiments to study processes such as cell migration, proliferation and differentiation. For example, labeling Cyanine5-NHS on cell membranes can track the movement of cells in vivo or in a petri dish.

3. Gene expression analysis: Cyanine5-NHS can be used to label DNA or RNA probes for gene expression analysis and in situ hybridization experiments. For example, in an in situ hybridization assay, Cyanine5-NHS can be labeled on a probe for a specific gene and used to detect the expression of that gene in a tissue or cell.

4. Tumor labeling: Cyanine5-NHS can be used in tumor labeling experiments to study the process of tumor growth and metastasis. For example, labeling Cyanine5-NHS on anti-tumor drugs can track the distribution and efficacy of the drugs in tumor tissues.

5. Drug delivery studies: Cyanine5-NHS can be used to study drug delivery and release processes. For example, labeling Cyanine5-NHS on nanoparticles or drug-carrying systems can track the distribution and release kinetics of drugs in vivo.

In conclusion, Cyanine5-NHS has a wide range of applications in the field of biological sciences and can be used in experiments such as immunofluorescence staining, cell tracing, gene expression analysis, tumor labeling and drug delivery studies.