Structure and properties of CY5-NHS Cyanine 5-active ester

Cyanine5-NHS is a fluorescent dye commonly used in biomarker and fluorescent staining experiments. It is an azo compound with an azo group and a nitrosyl chloride group.

The structure of Cyanine5-NHS is shown below:

CH3(CH2)2N(CH3)2 N=N C6H4CH=CHSO3

It is chemically known as N-hydroxysuccinimide ester of indocarbocyanine.Cyanine5-NHS is a fluorescent dye that absorbs and emits red light with high fluorescence quantum yield and photostability.

Key properties of Cyanine5-NHS include:

1. Fluorescence properties: Cyanine5-NHS has maximum absorption and emission peaks at light wavelengths in the range of 620-650 nanometers, so it can be used for red fluorescent staining.

2. Water solubility: Cyanine5-NHS is readily soluble in water and some organic solvents such as DMSO and methanol.

3. Chemical reactivity: The nitrosyl chloride group in Cyanine5-NHS has high reactivity and can condense with substances containing amino groups to form stable amide bonds.

4. Biocompatibility: Cyanine 5-NHS is relatively friendly to living organisms and can be labeled in cells and tissues for fluorescence microscopy observation and analysis.

In conclusion, Cyanine5-NHS is a commonly used red fluorescent dye with good fluorescence properties and chemical reactivity for biomarker and fluorescent staining experiments.