Differences in solubility and application areas between CY3 and sulfonated CY3

CY3 and sulfonated CY3 (Sulfo-CY3) are two commonly used fluorescent dyes that differ in solubility as follows:

1. Solubility of CY3:

   - CY3 is an organic dye with relatively poor solubility.CY3 usually needs to be dissolved using an organic solvent such as dimethyl sulfoxide or dimethylformamide to obtain a fluorescent dye solution that can be used in experiments. Because of its organic solvent solubility, CY3 is commonly used in fields such as organic synthesis and medicinal chemistry.

2. Solubility of sulfonated CY3 (Sulfo-CY3):

   - Sulfonated CY3 (Sulfo-CY3) is the sulfonate form of CY3 with better water solubility. It dissolves quickly in water and forms a stable aqueous solution, thus providing easier use and handling. The introduction of the sulfonate functional group gives Sulfo-CY3 better water solubility and fluorescence stability.

In summary, there is a clear difference in solubility between CY3 and sulfonated CY3. CY3 has poor solubility and requires the use of organic solvents to dissolve, and is commonly used in fields such as organic synthesis and medicinal chemistry. Sulfonated CY3 (Sulfo-CY3), on the other hand, has better water solubility and can be quickly dissolved in water and form a stable aqueous solution, providing easier use and handling. Therefore, the choice of which dye to use depends on specific experimental needs and conditions, such as the need for water solubility and ease of handling.

   - The application of CY3 is mainly focused on the fields of organic synthesis and medicinal chemistry. It is commonly used to label proteins, peptides, nucleic acids and small molecule compounds for their visualization and localization studies.CY3 has good stability and is suitable for long time storage and experimental operation.

   - The applications of sulfonated CY3 are mainly focused on biology and biomedical research. Due to its better water solubility and fluorescence stability, Sulfonated CY3 is commonly used to label biological macromolecules such as proteins, peptides, and nucleic acids for their localization, tracking and visualization. Sulfonated CY3 can also be used for cell labeling, immunohistochemistry and other aqueous phase experiments.

In summary, there are obvious differences between CY3 and sulfonated CY3 in terms of solubility and application.CY3 is suitable for organic synthesis and medicinal chemistry, etc. It has poor solubility and often requires the use of organic solvents. Sulfonated CY3 is suitable for biology and biomedical research, has better water solubility, and can be used for aqueous phase experiments and labeling of biomolecules. The choice of which dye to use depends on specific experimental needs and conditions, such as solubility requirements and type of experiment.