Cy3 can be applied to click reactions Cyanine 3-alkynes Cyanine 3-azides

Click reaction is a specific chemical reaction used to label molecules containing specific functional groups in biological molecules or cells.CY3 is commonly used to label molecules containing an alkyne functional group, causing them to undergo a click reaction with a dose containing an azobenzene functional group, resulting in the formation of a stable covalent linkage.CY3 is also used to label molecules containing an azobenzene functional group, resulting in a stable covalent linkage.

The steps for clicking on a response are as follows:

Marker preparation: Covalent binding of molecules containing alkyne functional groups to CY3. This can be accomplished by a chemical reaction (e.g., NHS ester reaction) or an enzyme-catalyzed coupling reaction. The introduction of CY3 into the molecule causes the molecule to bear a fluorescent label.

Click reaction: Reaction of a dose containing an azobenzene functional group with a labeled molecule. The click reaction is usually copper-catalyzed and forms a stable triazacyclic linkage by reacting the alkynyl groups of the two functional groups with the azobenzene functional group.

Visualization: After the click reaction is complete, the fluorescent signal of the labeled material can be observed by viewing the sample using a fluorescence microscope or other fluorescence imaging instrument.The fluorescence of CY3 emits a red light, so the marker can be located and visualized by its red fluorescence.

Click reactions can be used to label proteins, peptides, nucleic acids and other biomolecules for their localization, tracking and visualization. In biology and biomedical research, click reactions combined with CY3 fluorescent markers can be used in applications such as cellular tracing, protein interaction studies and disease labeling.