Polyethylene glycol-biotin RB-PEG-Biotin application MW:5000 In situ hybridization Biotin affinity purification

Rhodamine-PEG-Biotin is a compound with fluorescent properties and biotin structure, which has a wide range of applications in biomolecular labeling and bioanalysis.

In terms of biomolecular labeling, Rhodamine-PEG-Biotin binds to biotin and specifically binds to amphiphiles (e.g., amphiphile-labeled proteins or amphiphile resins) by affinity. This binding can be used for purification, detection and localization of biomolecules. For example, binding Rhodamine-PEG-Biotin to an antibody allows for the preparation of fluorescently labeled antibodies for immunofluorescent staining in immunocytochemistry to visualize the location of a target molecule in a cell or tissue.

In bioanalysis, Rhodamine-PEG-Biotin can be used in techniques such as fluorescence immunoassay, fluorescence in situ hybridization, and in situ PCR labeled with fluorescent dyes. It can act as a fluorescent signal generator and bind to target molecules for quantitative or qualitative analysis by fluorescence detection systems. For example, Rhodamine-PEG-Biotin labeled onto a DNA probe can be used in in situ hybridization techniques to observe the distribution of the target DNA in the cell nucleus by fluorescence microscopy to study biological processes such as gene expression and mutation.

In addition, Rhodamine-PEG-Biotin can be used in biotin affinity chromatography, biotin affinity purification and other technologies. By binding Rhodamine-PEG-Biotin to target proteins or structural domains, its purification and enrichment can be achieved.

In summary, Rhodamine-PEG-Biotin has a wide range of applications in biomolecular labeling and bioanalysis, and can be used in the fields of immunostaining, in situ hybridization, quantitative fluorescence analysis and biotin affinity purification.