Cyanine5 in biomarkers Cyanine dye CY5 Gene expression markers Protein interactions

Cyanine5 (Cy5) is a commonly used red fluorescent dye with a wide range of applications in biomarkers. The following are the applications of Cy5 in biomarkers:

1. Immunofluorescent labeling:

Cy5 can be conjugated to antibodies or antigens for immunofluorescent labeling. For example, in immunohistochemistry, Cy5 can be labeled on an antibody that binds the antibody to a protein or cell to be tested. By detecting the fluorescent signal of Cy5, the location and expression level of the target protein or cell in the sample to be tested can be determined.

2. Gene expression markers:

Cy5 can bind to DNA or RNA for gene expression labeling. For example, in in situ hybridization or FISH (fluorescence in situ hybridization), Cy5 can be labeled on a DNA or RNA probe that hybridizes with a specific sequence in the sample to be tested. By detecting the fluorescent signal of Cy5, the location and expression level of the target sequence in the sample to be tested can be determined.

3. Protein interaction studies:

Cy5 can be used to study protein interactions. For example, in a protein co-precipitation experiment, Cy5 can be labeled on a protein that is co-precipitated with other proteins in the sample to be tested. By detecting the fluorescent signal of Cy5, the protein that interacts with the Cy5-labeled protein can be identified.

4. Cell tracing and imaging:

Cy5 can be used in cell tracing and imaging studies. For example, in cell labeling and tracking, Cy5 can be labeled on specific structures or targets within a cell, and by detecting the fluorescent signal of Cy5, cell tracking and imaging can be achieved.

In conclusion, Cy5 has a wide range of applications as a red fluorescent dye in biomarkers. By binding to antibodies, DNA, RNA or other biomolecules, Cy5 enables the labeling and study of proteins, gene expression, protein interactions and cells. These applications can be used in immunological studies, gene expression analysis, protein studies and cell imaging, providing an important tool for biological research and medical diagnosis.