Application of CY3-NHS to biosensors Cyanine3-activated ester Detection of concentrations and changes in

Cyanine3-NHS (CY3-NHS) is widely used in biosensors to detect and analyze the presence and changes of biomolecules. The following are the applications of CY3-NHS in biosensors:

1. Protein sensors:

CY3-NHS can bind to specific proteins for protein sensor construction. For example, in a surface plasmon resonance sensor (SPR), CY3-NHS can be labeled on an antibody, which binds the antibody to the protein to be tested. By detecting changes in the fluorescence signal or SPR signal of CY3-NHS, the presence and concentration of the protein to be tested can be determined.

2. DNA sensors:

CY3-NHS can bind to DNA fragments for the construction of DNA sensors. For example, in a fluorescence resonance energy transfer (FRET) sensor, CY3-NHS can be labeled on a DNA fragment to form a FRET pair with another donor or acceptor dye. When the target DNA binds to the CY3-NHS-labeled DNA fragment, the intensity and efficiency of the FRET signal changes, allowing the presence and concentration of the target DNA to be detected and quantified.

3. Cellular sensors:

CY3-NHS can bind to molecules on the cell surface for the construction of cellular sensors. For example, in the detection of cell surface receptors, CY3-NHS can be labeled on a ligand, which binds the ligand to the receptors on the cell surface. By detecting the fluorescence signal of CY3-NHS, the presence and distribution of the cell surface receptor can be determined, thus enabling the analysis of cell signal transduction.

In conclusion, CY3-NHS has a wide range of applications as a fluorescent dye in biosensors. By binding to proteins, DNA fragments or cell surface molecules, CY3-NHS can realize the detection, quantitative analysis and signal transduction analysis of biomolecules. These applications can be used in protein sensors, DNA sensors and cell sensors, providing important tools for biological research, medical diagnosis and drug screening.