CY3-active ester on flow cytometry Cyanine3-NHS cell labeling Cycle analysis

Cyanine3-NHS (CY3-NHS) can be used as a fluorescent dye in flow cytometry for cell labeling and flow cytometric analysis. The following are applications of CY3-NHS in flow cytometry:

1. Cell surface labeling:

CY3-NHS can react with amino groups on the cell surface for fluorescent labeling of cells. For example, in flow cytometric analysis, CY3-NHS can be combined with specific proteins or markers on the cell surface to achieve quantitative and localization analysis of cell surface molecules. By detecting the fluorescent signal of CY3-NHS, different types or states of cells on the cell surface can be recognized and distinguished.

2. Intracellular labeling:

CY3-NHS can also be used for intracellular fluorescent labeling. For example, in intracellular molecular localization studies, CY3-NHS can be conjugated to specific proteins or molecules to achieve the analysis of their localization and distribution within the cell. By detecting the fluorescence signal of CY3-NHS through the fluorescence channel of the flow cytometer, the analysis of the localization and number of intracellular molecules can be realized.

3. Cell analysis:

CY3-NHS labeled cells can be analyzed by flow cytometry. For example, in cell cycle analysis, CY3-NHS can be combined with DNA to enable analysis of the DNA content and cell cycle of the cells. By measuring the intensity of the fluorescent signal of CY3-NHS, the DNA content of the cells can be determined and the ratio and distribution of the cells in different cell cycle stages can be further analyzed.

In conclusion, CY3-NHS has a wide range of applications as a fluorescent dye in flow cytometry. By binding to molecules on the cell surface or intracellularly, CY3-NHS enables fluorescent labeling and quantitative analysis of cell surface molecules and intracellular molecules. These applications can be used for cell surface labeling, intracellular molecule localization and analysis, and cell cycle analysis, providing an important tool for cell biology, immunology, and disease research.