Application of the cyanine dye CY3 in flow cytometry Rapid detection of

The cyanine dye CY3 (Cyanine 3) is also widely used in Flow Cytometry. Flow cytometry is a technique used to analyze and sequence cells by labeling them with a fluorescent dye, which, in combination with the lasers and detectors of a flow cytometer, allows for rapid detection and analysis of cells. The following are applications of the flower cyanine dye CY3 in flow cytometry and corresponding examples:

1. Cell surface labeling:

The cyanine dye CY3 can be used as a fluorescent dye for cell surface labeling. By labeling CY3 on an antibody that binds to a specific antigen on the cell surface, the expression of cell surface molecules can be detected and analyzed in flow cytometry. For example, labeling CY3 on a CD3 antibody can be used to detect and analyze the expression level of the surface CD3 receptor of T cells.

2. Intracellular staining:

The flower cyanine dye CY3 can also be used for intracellular staining. By labeling CY3 on a molecular probe, such as a DNA, RNA, or protein probe, a target molecule can be labeled within the cell and the molecular level within the cell can be detected and analyzed by flow cytometry. For example, labeling CY3 on a DNA probe can be used to detect and analyze the chromosome number and DNA content of a cell.

3. Cell sorting and sequencing:

The cyanine dye CY3 is also used in flow cytometry for cell sorting and sequencing. By labeling CY3 on a specific antibody or probe, cells labeled with a specific brightness of fluorescence can be selected for sorting and isolation. For example, by labeling CY3 on a cell surface CD4 antibody, CD4-positive cells can be sorted and isolated for subsequent experiments or applications.

In conclusion, the applications of the cyanine dye CY3 in flow cytometry include cell surface labeling, intracellular staining and cell sorting. By labeling CY3 on specific antibodies or probes, the detection, analysis and sorting of cell surface or intracellular molecules can be achieved, providing a powerful tool for cell biology research and clinical diagnosis.