FITC-PEG-Biotin Applications Polyethylene Glycol-Biotin MW:2000 Protein interactions Biotin-affinity system Affinity purification

FITC-PEG-Biotin is a compound that combines the fluorescent dye FITC with PEG and biotin (Biotin). It has a wide range of applications in biological research and medical applications:

1. Immunostaining: FITC-PEG-Biotin can be used for immunostaining to detect and localize the distribution and expression of specific antigens or molecules in cells or tissues. First, FITC-PEG-Biotin is conjugated to an antibody to form a FITC-PEG-Biotin-antibody complex. Then, the complex is bound to the target antigen in the cell or tissue, and the FITC tag is localized to the specific antigen by the biotin-affinity system. Finally, the intensity and position of the FITC signal is observed and analyzed using fluorescence microscopy to obtain information about the antigen.

2. Protein interactions: FITC-PEG-Biotin can be used to study protein interactions. FITC-PEG-Biotin-protein complexes are formed by binding FITC-PEG-Biotin to target proteins. The complex is then captured using an affinity protein or affinity resin to isolate and characterize other proteins that interact with the target protein.

3. Cell surface receptor detection: FITC-PEG-Biotin can be used to detect and study cell surface receptors. The ligand-FITC-PEG-Biotin complex is formed by binding FITC-PEG-Biotin to an appropriate ligand. The complex is then bound to the receptor on the cell surface and the FITC label is localized to the receptor using the biotin-affinity system. Using techniques such as flow cytometry or fluorescence microscopy, the intensity and location of the FITC signal can be detected and analyzed to obtain information about the cellular receptor.

4. Affinity purification: FITC-PEG-Biotin can be used to affinity purify specific proteins or biomolecules. A FITC-PEG-Biotin-protein complex is formed by binding FITC-PEG-Biotin to a target protein in a cell extract or other mixture. The complex is then captured using an affinity protein or affinity resin to isolate and purify the target protein.

In conclusion, FITC-PEG-Biotin, as a fluorescently labeled polyethylene glycol modifier, can be used in the fields of immunostaining, protein interactions, cell surface receptor detection and affinity purification. It provides a flexible and reliable method for studying biological processes such as localization, interaction and expression of biomolecules.