Structure and properties of CY7-MAL Cyanine7-Mal Thioether bonds

Cyanine7-Mal is a Cyanine7 dye with a maleimide (Mal) functional group. Its chemical structure is shown below:

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H3C--CH=CH--N(CH3)2--C6H4--CH=CH--C6H4--N(CH3)2--CH=CH--C6H4--CH=CH--C6H4--Mal

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Cyanine7-Mal has several features and properties:

1. Reactivity: The maleimide (Mal) functional group on Cyanine7-Mal is reactive and can be covalently coupled to compounds containing sulfhydryl groups (-SH). This coupling reaction, often referred to as the maleimide reaction, connects Cyanine7-Mal to other molecules through the formation of thioether bonds.

2. Optical properties: Cyanine 7-Mal is strongly fluorescent, with a maximum excitation wavelength of about 750 nm and a maximum emission wavelength of about 773 nm, which causes it to emit fluorescence in the near-infrared spectral range, which is favorable for optical imaging and analysis in living organisms.

3. Solubility: Cyanine 7-Mal has good solubility in a number of organic solvents (e.g. methanol, dimethyl sulfoxide, etc.) and water. This makes it have a better application prospect in biomolecular labeling and analysis.

4. Stability: Cyanine 7-Mal has good stability and can be stored for long periods of time and maintain its fluorescent properties under appropriate conditions. It is also highly stable to light and heat, making it suitable for extended use in optical imaging and analysis.

In conclusion, Cyanine7-Mal is a Cyanine7 dye with a maleimide functional group that possesses reactivity, optical properties, solubility and stability. It can be covalently coupled with compounds containing sulfhydryl groups for labeling and analysis of biomolecules. Due to the near-infrared fluorescence properties of Cyanine7-Mal, it can be used for biomedical imaging under lower background signal conditions, improving imaging sensitivity and resolution.