Structure and properties of CY7-NHS Cyanine7-active ester

Cyanine7-NHS is a Cyanine7 dye with an N-hydroxybutanediylimino (NHS) functional group. Its chemical structure is shown below:

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H3C--CH=CH--N(CH3)2--C6H4--CH=CH--C6H4--N(CH3)2--CH=CH--C6H4--CH=CH--C6H4--NHS

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Cyanine 7-NHS has several features and properties:

1. Reactivity: The NHS functional group on Cyanine7-NHS is reactive and can be covalently coupled to compounds containing amino (-NH2) or other nucleophilic functional groups. This coupling reaction, often referred to as the NHS reactive ester reaction, links Cyanine7-NHS to other molecules by forming amide bonds.

2. Optical properties: Cyanine 7-NHS has strong fluorescent properties, with a maximum excitation wavelength of about 750 nm and a maximum emission wavelength of about 773 nm, which makes it emit fluorescence in the near-infrared spectral range, which is favorable for optical imaging and analysis in organisms.

3. Solubility: Cyanine 7-NHS has good solubility in a number of organic solvents (e.g. methanol, dimethyl sulfoxide, etc.) and water. This makes it has a better application prospect in biomolecular labeling and analysis.

4. Stability: Cyanine 7-NHS has good stability and can be stored for long periods of time and maintain its fluorescent properties under appropriate conditions. It is also highly stable to light and heat, making it suitable for long term use in optical imaging and analysis.

5. For protein labeling applications: Cyanine7-NHS can react with the amino group (-NH2) in proteins to label them. This gives Cyanine7-NHS a wide range of applications in protein labeling and analysis.

In summary, Cyanine7-NHS is a Cyanine7 dye with an NHS functional group that possesses reactivity, optical properties, solubility and stability. It can be covalently coupled with compounds containing amino or other nucleophilic functional groups for labeling and analysis of biomolecules. Due to the near-infrared fluorescence properties of Cyanine7-NHS, it can be used for biomedical imaging under lower background signal conditions, improving imaging sensitivity and resolution.