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Cyanine7 on fluorescent staining of cells Membrane labeling Sensitivity

The application of Cyanine7 in cell fluorescence staining is mainly used to study the morphology, structure and function of cells by labeling it on antibodies or molecular probes. Below are some applications of Cyanine7 in cell fluorescence staining:

 

1. Immunofluorescence staining: Cyanine7 is labeled on specific antibodies and used for immunofluorescence staining to observe the localization and expression of specific proteins in cells. For example, Cyanine7 is labeled on antibodies and used to stain DNA in the nucleus of cells to study the structure and composition of chromosomes.

 

2. Cytoplasmic Staining: Cyanine7 is labeled on specific antibodies and used for cytoplasmic staining to visualize specific organelles or structures within cells. For example, Cyanine7 is labeled on an antibody against microtubule proteins and used to stain microtubules in cells to study the dynamics of the cytoskeleton.

 

3. Membrane labeling: Cyanine7 is labeled on specific antibodies or membrane-bound ligands for labeling of cell membranes to study the structure and function of cell membranes. For example, Cyanine7 is labeled on membrane-anchored antibodies for staining specific proteins on cell membranes to study cell adhesion and cell signaling.

 

4. Chromosome labeling: Cyanine7 is labeled on specific DNA probes for chromosome labeling to study the structure and function of chromosomes. For example, Cyanine7 is labeled on a DNA probe for fluorescence in situ hybridization, which is used to stain specific chromosomal regions within the nucleus to detect gene expression and genetic variation.

 

In conclusion, Cyanine7 has a wide range of applications in cytofluorescence staining and can be used in immunofluorescence staining, cytoplasmic staining, membrane labeling and chromosome labeling experiments. By labeling Cyanine7 on specific molecules, high-resolution observation and quantitative analysis of specific intracellular structures and molecules can be achieved. Due to the near-infrared fluorescence property of Cyanine7, it can be used for cell fluorescence imaging under the condition of lower background signal, improving the sensitivity and resolution of imaging.

 

 

Related Products

Cyanine CY7-carboxylic acid dyes  CY7-COOH

Cyanine CY7-Amino  CY7-NH2

Cyanine dyes CY7-active ester  CY7-NHS

Cyanine dye CY7-Maleimide  CY7-MAL


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