Cyanine5.5 in flow cytometry CY5.5 intracellular labeling

Cyanine 5.5 (Cy5.5) is widely used in flow cytometry for cell phenotyping, cell detection and cell sorting. Its longer wavelength and higher fluorescence stability make it ideal for flow cytometry. The following are applications of Cy5.5 in flow cytometry:

1. Cell surface labeling:

Labeling Cy5.5 on an antibody can be used for cell surface labeling. For example, labeling Cy5.5 on a CD45 antibody can be used for surface labeling for detection of white blood cells. This application can be used for cell phenotyping, immune cell detection, etc.

2. Cell viability assay:

Labeling Cy5.5 on a dye or probe can be used to detect cell viability. For example, labeling Cy5.5 on a cell permeability molecule, such as a cell membrane integrity dye, can assess cell viability and survival. Such applications can be used for cytotoxicity testing, drug screening, and cell viability assays.

3. Intracellular labeling:

Labeling Cy5.5 on cell permeable molecules or organelle specific molecules can be used for intracellular labeling. For example, labeling Cy5.5 on a mitochondrial dye allows observation of the distribution and number of mitochondria within a cell. This application can be used to study organelle function, metabolism, and kinetics.

4. Cell sorting:

The use of Cy5.5 labeled antibodies can be combined with cell sorting in flow cytometry. For example, Cy5.5 is labeled on a specific antibody to sort out specific types of cells by flow cytometry. This application can be used to isolate and purify specific subpopulations of cells, helping researchers with subsequent cell function and analysis.

In summary, Cyanine5.5 can be used in flow cytometry for cell surface labeling, cell viability assays, intracellular labeling and cell sorting. By labeling Cy5.5 on specific antibodies, dyes or probes, localization, tracking and fluorescent labeling of cells can be achieved. This application can help researchers with cell phenotyping, cell activity detection and cell purification. By combining the techniques of flow cytometry, sorting and purification of specific cell subpopulations can be achieved, providing an important tool for further cellular research.

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Cyanine CY5.5-carboxylic acid dyes  CY5.5-COOH

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Cyanine dye CY5.5-Active ester  CY5.5-NHS