CY5.5 on fluorescence resonance energy transfer (FRET) Cyanine5.5 Cell signaling Molecular dynamics

Cyanine5.5 (Cy5.5) plays an important role in fluorescence resonance energy transfer (FRET), a phenomenon in which energy is transferred through proximity interactions, and is commonly used in the study of protein interactions, cell signaling, and molecular dynamics. The following are the applications of Cy5.5 in FRET:

1. Protein interaction studies:

Conjugating Cy5.5 and another fluorescent dye (e.g., Cy3) to two proteins can be used to study protein interactions. For example, when Cy5.5 is labeled on one protein and Cy3 is labeled on the other, and the two proteins come into proximity through an interaction, the fluorescence energy of Cy3 is transferred to Cy5.5, producing a FRET signal. This application could help researchers understand the dynamics, spatial structure and function of protein interactions.

2. Molecular signaling studies:

Binding Cy5.5 to a signaling molecule can be used to study intracellular signaling processes. For example, Cy5.5 is labeled on a signaling molecule that generates a FRET signal with another fluorescent dye (e.g. Cy3) when binding or activation occurs in a specific signaling pathway. By observing the intensity and kinetic changes of the FRET signal, the localization, dynamic distribution, and changes in activity of the signaling molecule can be understood.

3. Fluorescent probe design:

The fluorescence of Cy5.5 is utilized for FRET probe design. For example, Cy5.5 is conjugated with a certain fluorescent moiety (e.g., NBD) to form a FRET pair. When a specific molecule is conjugated to this probe, a fluorescence resonance energy transfer occurs, causing Cy5.5 to emit a fluorescent signal. This application can be used to detect the presence, change and activity of specific molecules.

In conclusion, Cyanine5.5 can be used in fluorescence resonance energy transfer (FRET) to study protein interactions, cell signaling and molecular dynamics. By combining Cy5.5 with another fluorescent dye or signaling molecule, the observation and analysis of FRET phenomena can be achieved. This application can help researchers to understand the dynamics of protein interactions, the activity of signaling pathways, and changes in molecules. By designing suitable fluorescence resonance energy transfer probes, detection and monitoring of specific molecules can be realized.

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Cyanine CY5.5-carboxylic acid dye  CY5.5-COOH

Cyanine dye CY5.5-Amino  CY5.5-NH2

Cyanine dye CY5.5-Active ester  CY5.5-NHS