Fluorescent Properties of CY5 and Sulfonated CY5 and Fluorescence Instruments for

CY5 and sulfonated CY5 (Sulfo-CY5) are two commonly used fluorescent dyes that exhibit the following fluorescent properties

   - CY5 and sulfonated CY5 are both long-wavelength red fluorescent dyes with a maximum absorption wavelength of about 649 nm and a maximum emission wavelength of about 670 nm.They have high fluorescence intensity and long fluorescence lifetimes, and are suitable for use in fluorescence microscopy and other fluorescence imaging techniques.

   - CY5 and sulfonated CY5 have high fluorescence stability and are not easily affected by light and environmental conditions. Their fluorescence signals are long-lasting, facilitating prolonged observation and recording.

   - CY5 and sulfonated CY5 are suitable for use in various fluorescence imaging instruments, such as fluorescence microscopes, flow cytometers, and fluorescent enzyme markers.

   - For fluorescence microscopy, common excitation sources suitable for CY5 and sulfonated CY5 are lasers or xenon lamps. The excitation wavelength is usually in the range of 640-650 nm to excite the fluorescence of CY5 and sulfonated CY5 dyes.

   - In flow cytometry, the commonly used excitation wavelength is 633 nm, which is suitable for excitation of CY5 and sulfonated CY5 fluorescence.

   - For fluorescent enzyme markers, the commonly used excitation wavelength is 640 nm, which is suitable for fluorescence detection of CY5 and sulfonated CY5.

In summary, CY5 and sulfonated CY5 have high fluorescence intensity and stability, and are suitable for use in a wide range of fluorescence imaging instruments, such as fluorescence microscopes, flow cytometers and fluorescent enzyme markers. Their fluorescence properties make them ideal for studying the localization, tracking and visualization of biomolecules (e.g., proteins, nucleic acids).