Cyanine3-active ester in DNA microarray technology CY3-NHS gene mutation detection

Cyanine3-NHS (CY3-NHS) is often used as a fluorescent dye in DNA microarray technology for detecting and quantitatively analyzing the presence and changes in DNA molecules. The following are applications of CY3-NHS in DNA microarray technology:

1. DNA hybridization:

CY3-NHS can be combined with DNA fragments or primers for DNA hybridization on a DNA chip. For example, in a gene expression microarray, CY3-NHS can be labeled on a cDNA in an RNA sample, and the cDNA can be hybridized to a probe sequence on the DNA chip. By detecting the fluorescent signal of CY3-NHS, the level of gene expression in the RNA sample can be determined and compared between different samples.

2. DNA testing:

CY3-NHS can be conjugated to specific DNA probes for detecting and quantitatively analyzing the presence and changes in DNA molecules. For example, in gene mutation detection, CY3-NHS can be labeled on a primer for the mutation site and the primer can be hybridized to the DNA sample to be tested. By detecting the fluorescence signal of CY3-NHS, the presence or absence of the mutation in the sample to be tested can be determined, and the type and frequency of the mutation can be further analyzed.

3. DNA sequencing:

CY3-NHS can be conjugated to DNA fragments or primers for fluorescent labeling in DNA sequencing technologies. For example, in Sanger sequencing, CY3-NHS can be labeled on a primer, and the primer is subjected to an amplification reaction with DNA to be tested and a fluorescent labeling reaction. Fluorescent labeling and sequencing of DNA fragments can be achieved by the fluorescent signal of CY3-NHS.

In conclusion, CY3-NHS has important applications as a fluorescent dye in DNA microarray technology. By combining with DNA fragments or primers, CY3-NHS can realize fluorescent labeling, detection and quantitative analysis of DNA molecules. These applications can be used in gene expression microarrays, gene mutation detection and DNA sequencing, providing an important tool for gene function research, disease diagnosis and personalized medicine.