Cyanine dye CY3-carboxylate for biosensor applications Cyanine 3-COOH Cellular activity Pathogens

CY3-COOH has important applications in biosensors. A biosensor is a device capable of detecting and measuring biomolecules by labeling CY3-COOH on biomolecules.

1. Protein testing:

Detection of specific proteins can be achieved by labeling CY3-COOH on antibodies. For example, in clinical diagnosis, it is possible to label CY3-COOH on specific antibodies, and then bind these antibodies to target proteins in the samples to be tested, and finally, by detecting the fluorescent signals of CY3-COOH, it is possible to quantitatively detect the presence and concentration of the target proteins.

2. DNA testing:

Detection of specific DNA sequences can be achieved by labeling CY3-COOH on oligonucleotide probes. For example, in genetic diagnostics, CY3-COOH can be labeled on oligonucleotide probes complementary to the DNA fragments to be tested, and then these probes are bound to the DNA fragments in the samples to be tested, and finally, by detecting the fluorescent signals of the CY3-COOH, the presence and concentration of the target DNA can be quantitatively detected.

3. Cell activity assay:

The detection of cell activity can be achieved by labeling CY3-COOH on cell activity indicators. For example, in drug screening, CY3-COOH can be labeled on cell activity indicators, and then these indicators can be co-cultured with cells treated with the drug to be tested, and finally, by detecting the fluorescent signals of CY3-COOH, the effect of the drug on the activity of the cells can be assessed.

4. Pathogen detection:

The detection of pathogens can be achieved by labeling CY3-COOH on pathogen-specific probes. For example, in the field of food safety, CY3-COOH can be labeled on specific probes, and then these probes can be bound to pathogens in food samples to be tested, and finally the presence and concentration of pathogens can be quantified by detecting the fluorescent signal of CY3-COOH.

In conclusion, CY3-COOH as a fluorescent dye has a wide range of applications in biosensors. By labeling CY3-COOH on biomolecules, detection and quantitative analysis of proteins, DNA, cellular activity and pathogens can be achieved. These applications provide powerful tools for bioanalysis and bioassay.