Cyanine 3-COOH in DNA microarray technology Cyanine dye CY3-carboxy DNA sequencing Methylation analysis

CY3-COOH has important applications in DNA microarray technology DNA microarray is a technology used for high-throughput gene expression analysis by labeling CY3-COOH on DNA fragments. The following are the applications of CY3-COOH in DNA microarray technology and the corresponding examples:

1. Gene expression analysis:

By labeling CY3-COOH on cDNA or RNA, detection and analysis of gene expression can be achieved. For example, when studying the pathogenesis of a specific disease, CY3-COOH can be labeled on RNA from patients and normal controls, and then these samples can be hybridized with complementary probes on a DNA chip. Finally, by detecting the fluorescent signals of CY3-COOH, the expression levels of different genes can be quantitatively analyzed, thus understanding how genes are regulated in the disease.

2. DNA mutation detection:

Detection of DNA mutations can be achieved by labeling CY3-COOH on DNA fragments. For example, in genetic disease research, CY3-COOH can be labeled on DNA fragments from patients and normal controls, and then these samples can be hybridized with complementary probes on a DNA chip. Finally, by detecting the fluorescent signals of CY3-COOH, it is possible to detect and analyze mutations in genes, so as to understand the effects of mutations on diseases.

3. DNA methylation analysis:

Detection of DNA methylation status can be achieved by labeling CY3-COOH on DNA fragments. Methylation is a common DNA modification that is closely related to gene expression and disease occurrence. For example, in tumor research, CY3-COOH can be labeled on DNA fragments from tumor tissues and normal tissues, and then these samples can be hybridized with complementary probes on DNA microarrays. Finally, by detecting the fluorescent signals of CY3-COOH, the methylation levels of different genes can be analyzed, so that we can understand the role of methylation in tumorigenesis.

In conclusion, CY3-COOH, as a fluorescent dye, has a wide range of applications in DNA microarray technology. By labeling CY3-COOH on DNA fragments, detection and analysis of gene expression, DNA variation and DNA methylation status can be achieved. These applications provide powerful tools for gene expression analysis, genetic disease research and tumor research.