Cyanine dye CY3-carboxylate for flow cytometry Cyanine 3-COOH antigen quantification

CY3-COOH has important applications in flow cytometry. Flow cytometry is a high-throughput, high-sensitivity biological detection instrument that enables the analysis and classification of a large number of cells.CY3-COOH can be used to label antibodies, and by detecting their fluorescent signals, quantification and localization of cell surface antigens can be achieved. The following are applications of CY3-COOH on flow cytometry and corresponding examples:

1. Immunocyte staining:

By binding a CY3-COOH-labeled antibody to a specific antigen on the surface of a cell, the expression level of the cell surface antigen can be quantitatively analyzed in flow cytometry. For example, in cancer research, the CY3-COOH labeled antibody can be used to detect specific antigens on the surface of tumor cells, thereby assessing the number and expression level of tumor cells.

2. Cytotyping:

By binding CY3-COOH labeled antibodies to different cell surface antigens, different types of cells can be classified and typed in flow cytometry. For example, in immunological studies, CY3-COOH-labeled antibodies can be used to identify and classify different subpopulations of immune cells, such as lymphocytes, monocytes, and granulocytes.

3. Cell surface receptor studies:

By binding CY3-COOH-labeled antibodies to cell-surface receptors, the expression and activity of cell-surface receptors can be studied in flow cytometry. For example, in drug discovery, CY3-COOH-labeled antibodies can be used to assess the binding and activation effects of drugs on cell surface receptors.

4. Analysis of cellular functions:

By binding CY3-COOH-labeled antibodies to specific proteins within a cell, the function and activity of the cell can be studied in flow cytometry. For example, in cell signaling studies, CY3-COOH-labeled antibodies can be used to detect the expression and phosphorylation status of proteins associated with specific signaling pathways within the cell.

In conclusion, CY3-COOH as a fluorescent dye has a wide range of applications in flow cytometry. By binding CY3-COOH-labeled antibodies to cells or intracellular molecules, it is possible to quantify and localize cell surface antigens, and to study cell types, receptor expression, cell function and signaling. These applications provide powerful tools for cell analysis and functional studies.